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I'm not sure how I missed this one. Holly Bergevin's Perched Upon a Lily Pad is a CSS demo that shows off a 3 column layout with a flexible header, full length columns, horizontal navigation bar and complete descriptions of exactly how it all works contained within the page. It's very educational (I've learnt a trick or two just by reading it) and a great example of solid, cross-browser CSS design.

It's hosted as a guest demo over on Position is Everything, which now has four excellent layout demos.